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Protocol for Barcoding T Cells Combined with Timed Stimulations

Stimulation of naive T lymphocytes via the T cell receptor (TCR) induces distinct phosphorylation patterns that can be used to explore various signaling pathways within the cell. This protocol can be used to characterize different perturbations to the signaling pathways and the variations in time of...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Kulhanek, Kayla R., Myers, Darienne R., Ksionda, Olga, Vercoulen, Yvonne, Romero-Moya, Damia, Roose, Jeroen P.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580249/
https://ncbi.nlm.nih.gov/pubmed/33111105
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100067
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