An Optimized Protocol for Proximity Biotinylation in Confluent Epithelial Cell Cultures Using the Peroxidase APEX2

The peroxidase APEX2 has been used widely for proximity biotinylation and subsequent proteomics analyses. However, the poor membrane permeability of the biotin phenol substrate and the inhibitory effect of peroxide on the enzyme’s activity has hampered proximity labeling in certain cell culture syst...

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Vydáno v:STAR Protoc
Hlavní autoři: Tan, Benedict, Peng, Suat, Yatim, Siti Maryam J.M., Gunaratne, Jayantha, Hunziker, Walter, Ludwig, Alexander
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2020
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580243/
https://ncbi.nlm.nih.gov/pubmed/33111110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100074
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