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Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells
The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes...
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| Publicado no: | STAR Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8102175/ https://ncbi.nlm.nih.gov/pubmed/33997820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100506 |
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