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Processing Human Thymic Tissue for Single Cell RNA-Seq
Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34(+) progenitor and more differentiated CD34(–) fractions from post-natal thymic tissue to study thymopoiesis. CD34(+) cells represent &l...
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| 發表在: | STAR Protoc |
|---|---|
| Main Authors: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier
2020
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7580228/ https://ncbi.nlm.nih.gov/pubmed/33111122 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100090 |
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