Protocol for Immuno-Enrichment of FLAG-Tagged Protein Complexes

This protocol describes immunoprecipitation of proteins associated with FLAG-tagged recombinant proteins followed by mass spectrometry-based proteomics to identify the associated interactome components. FLAG epitope was chosen, because existing high-affinity monoclonal antibodies allow for sensitive...

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Vydáno v:STAR Protoc
Hlavní autoři: Valdez-Sinon, Arielle Nicole, Gokhale, Avanti, Faundez, Victor, Bassell, Gary Jonathan
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2020
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580096/
https://ncbi.nlm.nih.gov/pubmed/33111116
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100083
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