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Protocol for Immuno-Enrichment of FLAG-Tagged Protein Complexes
This protocol describes immunoprecipitation of proteins associated with FLAG-tagged recombinant proteins followed by mass spectrometry-based proteomics to identify the associated interactome components. FLAG epitope was chosen, because existing high-affinity monoclonal antibodies allow for sensitive...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7580096/ https://ncbi.nlm.nih.gov/pubmed/33111116 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100083 |
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