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Effects of Kifunensine on Production and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice Cell Culture Bioreactor

The production and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a model highly glycosylated therapeutic protein, in a transgenic rice cell suspension culture treated with kifunensine, a strong α-mannosidase I inhibitor, was studied in a 5 L bioreactor. A media exchange was perf...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: Macharoen, Kantharakorn, Li, Qiongyu, Márquez-Escobar, Veronica A., Corbin, Jasmine M., Lebrilla, Carlito B., Nandi, Somen, McDonald, Karen A.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7555773/
https://ncbi.nlm.nih.gov/pubmed/32962231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21186896
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