Purification, characterization, and N-glycosylation of recombinant butyrylcholinesterase from transgenic rice cell suspension cultures

Recombinant butyrylcholinesterase produced in a metabolically regulated transgenic rice cell culture (rrBChE) was purified to produce a highly pure (95%), active form of enzyme. The developed downstream process uses common manufacturing friendly operations including tangential flow filtration, anion...

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Veröffentlicht in:Biotechnol Bioeng
Hauptverfasser: Corbin, Jasmine M., Kailemia, Muchena J., Cadieux, C. Linn, Alkanaimsh, Salem, Karuppanan, Kalimuthu, Rodriguez, Raymond L., Lebrilla, Carlito B., Cerasoli, Douglas M., McDonald, Karen A., Nandi, Somen
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7341864/
https://ncbi.nlm.nih.gov/pubmed/29411865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26557
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