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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Single molecule (SM) microscopy is used in the study of dynamic molecular interactions of fluorophore labeled biomolecules in real time. However, fluorophores are prone to loss of signal via photobleaching by dissolved oxygen (O(2)). To prevent photobleaching and extend the fluorophore lifetime, oxy...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Messer, Ryan K., Lopez, Miguel A., Senavirathne, Gayan, Yoder, Kristine E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7552998/
https://ncbi.nlm.nih.gov/pubmed/31762446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59599
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