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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Single molecule (SM) microscopy is used in the study of dynamic molecular interactions of fluorophore labeled biomolecules in real time. However, fluorophores are prone to loss of signal via photobleaching by dissolved oxygen (O(2)). To prevent photobleaching and extend the fluorophore lifetime, oxy...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Messer, Ryan K., Lopez, Miguel A., Senavirathne, Gayan, Yoder, Kristine E.
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7552998/
https://ncbi.nlm.nih.gov/pubmed/31762446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59599
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