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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Single molecule (SM) microscopy is used in the study of dynamic molecular interactions of fluorophore labeled biomolecules in real time. However, fluorophores are prone to loss of signal via photobleaching by dissolved oxygen (O(2)). To prevent photobleaching and extend the fluorophore lifetime, oxy...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Autores principales: Messer, Ryan K., Lopez, Miguel A., Senavirathne, Gayan, Yoder, Kristine E.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7552998/
https://ncbi.nlm.nih.gov/pubmed/31762446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59599
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