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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Single molecule (SM) microscopy is used in the study of dynamic molecular interactions of fluorophore labeled biomolecules in real time. However, fluorophores are prone to loss of signal via photobleaching by dissolved oxygen (O(2)). To prevent photobleaching and extend the fluorophore lifetime, oxy...
Gorde:
| Argitaratua izan da: | J Vis Exp |
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| Egile Nagusiak: | , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
2019
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7552998/ https://ncbi.nlm.nih.gov/pubmed/31762446 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59599 |
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