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Expression and Purification of Nuclease-Free Protocatechuate 3,4-Dioxygenase for Prolonged Single-Molecule Fluorescence Imaging
Single-molecule (SM) microscopy is a powerful tool capable of visualizing individual molecules and events in real time. SM imaging may rely on proteins or nucleic acids labelled with a fluorophore. Unfortunately photobleaching of fluorophores leads to irreversible loss of signal, impacting the colle...
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| Pubblicato in: | Anal Biochem |
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| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6076860/ https://ncbi.nlm.nih.gov/pubmed/29932890 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2018.06.016 |
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