lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements

Massively parallel reporter assays (MPRAs) can simultaneously measure the function of thousands of candidate regulatory sequences (CRSs) in a quantitative manner. In this method, CRSs are cloned upstream of a minimal promoter and reporter gene, alongside a unique barcode, and introduced into cells....

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Gepubliceerd in:Nat Protoc
Hoofdauteurs: Gordon, M. Grace, Inoue, Fumitaka, Martin, Beth, Schubach, Max, Agarwal, Vikram, Whalen, Sean, Feng, Shiyun, Zhao, Jingjing, Ashuach, Tal, Ziffra, Ryan, Kreimer, Anat, Georgakopoulous-Soares, Ilias, Yosef, Nir, Ye, Chun Jimmie, Pollard, Katherine S., Shendure, Jay, Kircher, Martin, Ahituv, Nadav
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2020
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7550205/
https://ncbi.nlm.nih.gov/pubmed/32641802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-0333-5
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