Position of Deltaproteobacteria Cas12e nuclease cleavage sites depends on spacer length of guide RNA

Cas12e proteins (formerly CasX) form a distinct subtype of Class II type V CRISPR-Cas effectors. Recently, it was shown that DpbCas12e from Deltaproteobacteria and PlmCas12e from Planctomycetes can introduce programmable double-stranded breaks in mammalian genomes. Thus, along with Cas9 and Cas12a C...

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Detalhes bibliográficos
Publicado no:RNA Biol
Main Authors: Selkova, Polina, Vasileva, Aleksandra, Pobegalov, Georgii, Musharova, Olga, Arseniev, Anatolii, Kazalov, Maksim, Zyubko, Tatyana, Shcheglova, Nataliia, Artamonova, Tatyana, Khodorkovskii, Mikhail, Severinov, Konstantin, Fedorova, Iana
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2020
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Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7549622/
https://ncbi.nlm.nih.gov/pubmed/32564655
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2020.1777378
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