Generation of Human Neutrophils from Induced Pluripotent Stem Cells in Chemically Defined Conditions Using ETV2 Modified mRNA

This protocol describes a rapid and efficient feeder-, serum-, and xeno-free method for neutrophil generation from hiPSCs using ETV2 modified mRNA (mmRNA), which directs hematoendothelial programming of hiPSCs. Hematoendothelial progenitors were cultured with GM-CSF, FGF-2, and UM171 to expand myelo...

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Bibliografske podrobnosti
izdano v:STAR Protoc
Main Authors: Majumder, Aditi, Suknuntha, Kran, Bennin, David, Klemm, Lucas, Brok-Volchanskaya, Vera S., Huttenlocher, Anna, Slukvin, Igor
Format: Artigo
Jezik:Inglês
Izdano: Elsevier 2020
Teme:
Protocol
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7543976/
https://ncbi.nlm.nih.gov/pubmed/33043305
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100075
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