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Saturation transfer difference NMR on the integral trimeric membrane transport protein GltPh determines cooperative substrate binding

Saturation-transfer difference (STD) NMR spectroscopy is a fast and versatile method which can be applied for drug-screening purposes, allowing the determination of essential ligand binding affinities (K(D)). Although widely employed to study soluble proteins, its use remains negligible for membrane...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Hall, Jenny L., Sohail, Azmat, Cabrita, Eurico J., Macdonald, Colin, Stockner, Thomas, Sitte, Harald H., Angulo, Jesus, MacMillan, Fraser
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7536232/
https://ncbi.nlm.nih.gov/pubmed/33020522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-73443-z
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