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Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy

Determining nanoscale protein distribution via Photoactivated Localization Microscopy (PALM) mandates precise knowledge of the applied fluorophore’s blinking properties to counteract overcounting artifacts that distort the resulting biomolecular distributions. Here, we present a readily applicable m...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Platzer, René, Rossboth, Benedikt K., Schneider, Magdalena C., Sevcsik, Eva, Baumgart, Florian, Stockinger, Hannes, Schütz, Gerhard J., Huppa, Johannes B., Brameshuber, Mario
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7536177/
https://ncbi.nlm.nih.gov/pubmed/33020470
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-18726-9
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