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Selective monitoring of insulin secretion after CRISPR interference in intact pancreatic islets despite submaximal infection
Virus-mediated gene knockdown in intact pancreatic islets is technically challenging due to poor infection of the center of the islet. Because the cells that do not have knockdown have normal insulin secretion, measuring changes in insulin secretion after gene knockdown is challenging. We describe a...
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| Pubblicato in: | Islets |
|---|---|
| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Taylor & Francis
2020
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7527018/ https://ncbi.nlm.nih.gov/pubmed/32579048 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/19382014.2020.1752072 |
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