Selective monitoring of insulin secretion after CRISPR interference in intact pancreatic islets despite submaximal infection

Virus-mediated gene knockdown in intact pancreatic islets is technically challenging due to poor infection of the center of the islet. Because the cells that do not have knockdown have normal insulin secretion, measuring changes in insulin secretion after gene knockdown is challenging. We describe a...

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Foilsithe in:Islets
Main Authors: Shariati, Kaavian, Pappalardo, Zachary, Chopra, Deeksha G., Yiv, Nicholas, Sheen, Robin, Ku, Gregory
Formáid: Artigo
Teanga:Inglês
Foilsithe: Taylor & Francis 2020
Ábhair:
Research Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7527018/
https://ncbi.nlm.nih.gov/pubmed/32579048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/19382014.2020.1752072
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