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Quantification of Insoluble Protein Aggregation in Caenorhabditis elegans during Aging with a Novel Data-Independent Acquisition Workflow

We and others have shown that the aging process results in a proteome-wide accumulation of insoluble proteins. Knocking down genes encoding the insoluble proteins over 40% of the time results in an extension of the lifespan in C. elegans, suggesting that many of these proteins are key determinants o...

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Xie, Xueshu, Chamoli, Manish, Bhaumik, Dipa, Sivapatham, Renuka, Angeli, Suzanne, Andersen, Julie K., Lithgow, Gordon J., Schilling, Birgit
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2020
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7519758/
https://ncbi.nlm.nih.gov/pubmed/32831297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61366
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