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Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing

Precision genome engineering has dramatically advanced with the development of CRISPR/Cas base editing systems that include cytosine base editors and adenine base editors (ABEs). Herein, we compare the editing profile of circularly permuted and domain-inlaid Cas9 base editors, and find that on-targe...

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Dades bibliogràfiques
Publicat a:Nat Commun
Autors principals: Nguyen Tran, Minh Thuan, Mohd Khalid, Mohd Khairul Nizam, Wang, Qi, Walker, Jacqueline K. R., Lidgerwood, Grace E., Dilworth, Kimberley L., Lisowski, Leszek, Pébay, Alice, Hewitt, Alex W.
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7519688/
https://ncbi.nlm.nih.gov/pubmed/32978399
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-18715-y
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