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Comparison of CRISPR Genomic Tagging For Affinity-Purification and Endogenous Immunoprecipitation coupled with Quantitative MS to Identify the Dynamic AMPKα2 Interactome
Recent advances in genome editing technologies have enabled the insertion of epitope tags at endogenous loci with relative efficiency. We describe an approach for investigation of protein interaction dynamics of the AMP activated kinase complex AMPK using a catalytic subunit AMPKα2 (PRKAA2 gene) as...
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| Veröffentlicht in: | J Proteome Res |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7512655/ https://ncbi.nlm.nih.gov/pubmed/31398040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.9b00378 |
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