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Recombineering: Using Drug Cassettes to Knock out Genes in vivo

A ‘gene knockout’ or ‘knockout’ is a mutation that inactivates a gene function. These mutations are very useful for classical genetic studies as well as for modern techniques including functional genomics. In the past, knockouts of bacterial genes were often made by transposon mutagenesis. In this c...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Sawitzke, James A., Thomason, Lynn C., Bubunenko, Mikhail, Li, Xintian, Costantino, Nina, Court, Donald L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7511988/
https://ncbi.nlm.nih.gov/pubmed/24182919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-420067-8.00007-6
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