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Probing cellular processes with oligo-mediated recombination; using knowledge gained to optimize recombineering
Recombination with single-strand DNA oligonucleotides (oligos) in E. coli is an efficient and rapid way to modify replicons in vivo. The generation of a nucleotide alteration by oligo recombination provides novel assays for studying cellular processes. Single-strand exonucleases inhibit oligo recomb...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3046259/ https://ncbi.nlm.nih.gov/pubmed/21256136 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.01.030 |
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