Probing cellular processes with oligo-mediated recombination; using knowledge gained to optimize recombineering

Recombination with single-strand DNA oligonucleotides (oligos) in E. coli is an efficient and rapid way to modify replicons in vivo. The generation of a nucleotide alteration by oligo recombination provides novel assays for studying cellular processes. Single-strand exonucleases inhibit oligo recomb...

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Main Authors: Sawitzke, James A., Costantino, Nina, Li, Xin-tian, Thomason, Lynn C., Bubunenko, Mikhail, Court, Carolyn, Court, Donald L.
Format: Artigo
Jezik:Inglês
Izdano: 2011
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3046259/
https://ncbi.nlm.nih.gov/pubmed/21256136
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.01.030
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