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The segregation of Escherichia coli minichromosomes constructed in vivo by recombineering
Circularized regions of the chromosome containing the origin of replication, oriC, can be maintained as autonomous minichromosomes, oriC plasmids. We show that oriC plasmids containing precise, pre-determined segments of the chromosome can be generated by a simple in vivo recombineering technique. W...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3319274/ https://ncbi.nlm.nih.gov/pubmed/22252137 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2012.01.002 |
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