Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes

Post-mitotic cardiomyocytes have been considered to be non-permissive to precise targeted integration including homology-directed repair (HDR) after CRISPR/Cas9 genome editing. Here, we demonstrate that direct delivery of large amounts of transgene encoding guide RNA (gRNA) and repair template DNA v...

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Udgivet i:Sci Rep
Main Authors: Kohama, Yasuaki, Higo, Shuichiro, Masumura, Yuki, Shiba, Mikio, Kondo, Takumi, Ishizu, Takamaru, Higo, Tomoaki, Nakamura, Satoki, Kameda, Satoshi, Tabata, Tomoka, Inoue, Hiroyuki, Motooka, Daisuke, Okuzaki, Daisuke, Takashima, Seiji, Miyagawa, Shigeru, Sawa, Yoshiki, Hikoso, Shungo, Sakata, Yasushi
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7501291/
https://ncbi.nlm.nih.gov/pubmed/32948788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-72216-y
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