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Somatic Liver Knockout (SLiK): A quick and efficient way of generating liver-specific knockout mice using multiplex CRISPR/Cas9 gene editing
Somatic Liver Knockout (SLiK) is a method developed to rapidly generate a liver-specific knockout of one or several genes. This technique combines the strengths of CRISPR/Cas9 gene editing and hydrodynamic tail vein injection, a simple in vivo method for transfection of hepatocytes, to harness the p...
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| Publicado no: | Curr Protoc Mol Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7500866/ https://ncbi.nlm.nih.gov/pubmed/32150344 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.117 |
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