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Analysis of Brightness of a Single Fluorophore for Quantitative Characterization of Biochemical Reactions

[Image: see text] Intrinsic molecular brightness (MB) is a number of emitted photons per second per molecule. When a substrate labeled by a fluorophore and a second unlabeled substrate form a complex in solution, the MB of the fluorophore changes. Here we use this change to determine the equilibrium...

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Publicat a:J Phys Chem B
Autors principals: Bielec, Krzysztof, Bubak, Grzegorz, Kalwarczyk, Tomasz, Holyst, Robert
Format: Artigo
Idioma:Inglês
Publicat: American Chemical Society 2020
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7497653/
https://ncbi.nlm.nih.gov/pubmed/32059107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.0c00770
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