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Analysis of Brightness of a Single Fluorophore for Quantitative Characterization of Biochemical Reactions
[Image: see text] Intrinsic molecular brightness (MB) is a number of emitted photons per second per molecule. When a substrate labeled by a fluorophore and a second unlabeled substrate form a complex in solution, the MB of the fluorophore changes. Here we use this change to determine the equilibrium...
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| Publicado no: | J Phys Chem B |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Chemical
Society
2020
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7497653/ https://ncbi.nlm.nih.gov/pubmed/32059107 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.0c00770 |
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