Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture

Systemic and local posttraumatic responses are often monitored on mRNA expression level using quantitative real-time PCR (qRT-PCR), which requires normalisation to adjust for confounding sources of variability. Normalisation requests reference (housekeeping) genes stable throughout time and divergen...

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Vydáno v:Sci Rep
Hlavní autoři: Otto, Ellen, Köhli, Paul, Appelt, Jessika, Menzel, Stefanie, Fuchs, Melanie, Bahn, Alina, Graef, Frank, Duda, Georg N., Tsitsilonis, Serafeim, Keller, Johannes, Jahn, Denise
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2020
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7490670/
https://ncbi.nlm.nih.gov/pubmed/32929099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-71895-x
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