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Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

Detrusor smooth muscle (DSM) cells present within the urinary bladder wall ultimately facilitate urine storage and voiding. Preparation of the viable, fresh, and isolated DSM cells presents an important technical challenge whose achievement provides optimal cells for subsequent functional and molecu...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Malysz, John, Rovner, Eric S., Wake, Robert, Petkov, Georgi V.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7489995/
https://ncbi.nlm.nih.gov/pubmed/32065126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59884
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