Developing fluorescence sensor probe to capture activated muscle-specific calpain-3 (CAPN3) in living muscle cells

Calpain-3 (CAPN3) is a muscle-specific type of calpain whose protease activity is triggered by Ca(2+). Here, we developed CAPN3 sensor probes (SPs) to detect activated-CAPN3 using a fluorescence/Förster resonance energy transfer (FRET) technique. In our SPs, partial amino acid sequence of calpastati...

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Bibliographische Detailangaben
Veröffentlicht in:Biol Open
Hauptverfasser: Ojima, Koichi, Hata, Shoji, Shinkai-Ouchi, Fumiko, Oe, Mika, Muroya, Susumu, Sorimachi, Hiroyuki, Ono, Yasuko
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Company of Biologists Ltd 2020
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7489760/
https://ncbi.nlm.nih.gov/pubmed/32801165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.048975
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