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RNA–protein interaction mapping via MS2- or Cas13-based APEX targeting

RNA–protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA–protein interactions in living cells in an unbiased manner. We used two approaches to target the engineered peroxidase APEX2 to specific cellular RNAs for RNA-centered proximit...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Han, Shuo, Zhao, Boxuan Simen, Myers, Samuel A., Carr, Steven A., He, Chuan, Ting, Alice Y.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7486720/
https://ncbi.nlm.nih.gov/pubmed/32839320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2006617117
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