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Fast Protein Footprinting by X-ray Mediated Radical Trifluoromethylation

Synchrotron radiolysis generates hydroxyl radicals (•OH) that are successful footprinting reagents. Here, we describe a new reagent for the synchrotron platform, the trifluoromethyl radical (•CF(3)). The radical is produced by •OH displacement of •CF(3) from sodium triflinate (Langlois reagent). Upo...

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Bibliografske podrobnosti
izdano v:J Am Soc Mass Spectrom
Main Authors: Cheng, Ming, Asuru, Awuri, Kiselar, Janna, Mathai, George, Chance, Mark R., Gross, Michael L.
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7486011/
https://ncbi.nlm.nih.gov/pubmed/32255631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jasms.0c00085
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