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Organic Solvents for Enhanced Proteolysis of Stable Proteins for Hydrogen/Deuterium Exchange Mass Spectrometry

Protein digestion is a key challenge in mass spectrometry (MS)-based structural proteomics. Although using hydrogen deuterium exchange kinetics with mass spectrometry (HDX-MS) to interrogate high order structure of proteins is now established, it can be challenging for ß-barrel proteins, which are i...

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書目詳細資料
發表在:Anal Chem
Main Authors: Guo, Chunyang, Steinberg, Lindsey K., Henderson, Jeffrey P., Gross, Michael L.
格式: Artigo
語言:Inglês
出版: 2020
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7485609/
https://ncbi.nlm.nih.gov/pubmed/32867496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c02194
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