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A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences
To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive T-cell clones it is necessary to develop convenient and rapid cloning strategies for the generation of TCR expression constructs. Herein, we describe a pDONR™221 vector backbone allowing to generate Gateway™ com...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7480877/ https://ncbi.nlm.nih.gov/pubmed/32903281 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0238875 |
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