A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive T-cell clones it is necessary to develop convenient and rapid cloning strategies for the generation of TCR expression constructs. Herein, we describe a pDONR™221 vector backbone allowing to generate Gateway™ com...

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Publicat a:PLoS One
Autors principals: Kropp, Korbinian N., Schäufele, Tim J., Fatho, Martina, Volkmar, Michael, Conradi, Roland, Theobald, Matthias, Wölfel, Thomas, Wölfel, Catherine
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2020
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7480877/
https://ncbi.nlm.nih.gov/pubmed/32903281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0238875
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