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Seamless Ligation Cloning Extract (SLiCE) Cloning Method

SLiCE (Seamless Ligation Cloning Extract) is a novel cloning method that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of traditional cloning me...

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Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Zhang, Yongwei, Werling, Uwe, Edelmann, Winfried
Format: Artigo
Language:Inglês
Published: 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4672941/
https://ncbi.nlm.nih.gov/pubmed/24395368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-764-8_16
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