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A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis
BACKGROUND: Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses the components of the λ prophage Red/ET recombination system. This method facilitates restriction endonuclease cleavag...
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| Publié dans: | BMC Biotechnol |
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| Auteur principal: | |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4453199/ https://ncbi.nlm.nih.gov/pubmed/26037246 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0162-8 |
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