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A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis

BACKGROUND: Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses the components of the λ prophage Red/ET recombination system. This method facilitates restriction endonuclease cleavag...

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Bibliografski detalji
Izdano u:BMC Biotechnol
Glavni autor: Motohashi, Ken
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4453199/
https://ncbi.nlm.nih.gov/pubmed/26037246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0162-8
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