Dehydrogenase Binding Sites Abolish the “Dark” Fraction of NADH: Implication for Metabolic Sensing via FLIM

The fluorescence of dinucleotide NADH has been exploited for decades to determine the redox state of cells and tissues in vivo and in vitro. Particularly, nanosecond (ns) fluorescence lifetime imaging microscopy (FLIM) of NADH (in free vs bound forms) has recently offered a label-free readout of mit...

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Bibliographic Details
Published in:J Phys Chem B
Main Authors: Cao, Simin, Li, Haoyang, Liu, Yangyi, Wang, Mengyu, Zhang, Mengjie, Zhang, Sanjun, Chen, Jinquan, Xu, Jianhua, Knutson, Jay R., Brand, Ludwig
Format: Artigo
Language:Inglês
Published: 2020
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7477841/
https://ncbi.nlm.nih.gov/pubmed/32660250
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.0c04835
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