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Separating NADH and NADPH fluorescence in live cells and tissues using FLIM

NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form, NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced forms of both pyridine nucleotides are fluorescent in living cells but they cannot be distinguished, as they are spec...

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Detalhes bibliográficos
Main Authors: Blacker, Thomas S., Mann, Zoe F., Gale, Jonathan E., Ziegler, Mathias, Bain, Angus J., Szabadkai, Gyorgy, Duchen, Michael R.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Pub. Group 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4046109/
https://ncbi.nlm.nih.gov/pubmed/24874098
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms4936
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