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Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime

NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role in cellular antioxidant defence. In live cells and tissues, the intensity of their spectrally-identical autofluorescence, termed NAD(P)H, can...

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Pubblicato in:Bio Protoc
Autori principali: Blacker, Thomas S., Berecz, Tunde, Duchen, Michael R., Szabadkai, Gyorgy
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-Protocol 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5345742/
https://ncbi.nlm.nih.gov/pubmed/28286806
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2105
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