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Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments

Theoretical and experimental observations that catalysis enhances the diffusion of enzymes have generated exciting implications about nanoscale energy flow, molecular chemotaxis, and self-powered nanomachines. However, contradictory claims on the origin, magnitude, and consequence of this phenomenon...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Chen, Zhijie, Shaw, Alan, Wilson, Hugh, Woringer, Maxime, Darzacq, Xavier, Marqusee, Susan, Wang, Quan, Bustamante, Carlos
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7474647/
https://ncbi.nlm.nih.gov/pubmed/32817484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2006900117
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