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Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments

Theoretical and experimental observations that catalysis enhances the diffusion of enzymes have generated exciting implications about nanoscale energy flow, molecular chemotaxis, and self-powered nanomachines. However, contradictory claims on the origin, magnitude, and consequence of this phenomenon...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Chen, Zhijie, Shaw, Alan, Wilson, Hugh, Woringer, Maxime, Darzacq, Xavier, Marqusee, Susan, Wang, Quan, Bustamante, Carlos
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7474647/
https://ncbi.nlm.nih.gov/pubmed/32817484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2006900117
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