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Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments

Theoretical and experimental observations that catalysis enhances the diffusion of enzymes have generated exciting implications about nanoscale energy flow, molecular chemotaxis, and self-powered nanomachines. However, contradictory claims on the origin, magnitude, and consequence of this phenomenon...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Chen, Zhijie, Shaw, Alan, Wilson, Hugh, Woringer, Maxime, Darzacq, Xavier, Marqusee, Susan, Wang, Quan, Bustamante, Carlos
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7474647/
https://ncbi.nlm.nih.gov/pubmed/32817484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2006900117
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