Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates

Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate characterization and relatedness determination. However, standardized and harmonized workflows, e.g. for DNA extraction, are required to ensure robust and exchangeable WGS data. Data sharing between (inter)nationa...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Nouws, Stéphanie, Bogaerts, Bert, Verhaegen, Bavo, Denayer, Sarah, Piérard, Denis, Marchal, Kathleen, Roosens, Nancy H. C., Vanneste, Kevin, De Keersmaecker, Sigrid C. J.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7474065/
https://ncbi.nlm.nih.gov/pubmed/32887913
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-71207-3
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