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Creating a library of random transposon mutants in Leptospira
Generation of a random transposon mutant library is advantageous in Leptospira as site directed mutagenesis remains a challenge, especially in pathogenic species. This procedure is typically completed by transformation of Leptospira with a Himar1 containing plasmid via conjugation with Escherichia c...
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| Udgivet i: | Methods Mol Biol |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7473121/ https://ncbi.nlm.nih.gov/pubmed/32632861 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0459-5_8 |
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