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A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase

Deoxyribonucleoside triphosphates (dNTPs) are vital for the biosynthesis and repair of DNA. Their cellular concentration peaks during the S phase of the cell cycle. In non-proliferating cells, dNTP concentrations are low, making their reliable quantification from tissue samples of heterogeneous cell...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Purhonen, Janne, Banerjee, Rishi, McDonald, Allison E, Fellman, Vineta, Kallijärvi, Jukka
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7470940/
https://ncbi.nlm.nih.gov/pubmed/32573728
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa516
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