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Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules

Bacteria use surface-exposed, proteinaceous fibers called pili for diverse behaviors including horizontal gene transfer, surface sensing, motility, and pathogenicity. Visualization of these nanomachines and their activity in live cells has proved challenging largely due to their small size. Here, we...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Ellison, Courtney K., Dalia, Triana N., Dalia, Ankur B., Brun, Yves V.
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7461830/
https://ncbi.nlm.nih.gov/pubmed/31028374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0162-6
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