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Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules

Bacteria use surface-exposed, proteinaceous fibers called pili for diverse behaviors including horizontal gene transfer, surface sensing, motility, and pathogenicity. Visualization of these nanomachines and their activity in live cells has proved challenging largely due to their small size. Here, we...

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Bibliographic Details
Published in:Nat Protoc
Main Authors: Ellison, Courtney K., Dalia, Triana N., Dalia, Ankur B., Brun, Yves V.
Format: Artigo
Language:Inglês
Published: 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7461830/
https://ncbi.nlm.nih.gov/pubmed/31028374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0162-6
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