sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine

We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site. Among tested sgBEs, sgBE-SL4, in which deaminase is attached to the last stem-loop of sgRNA, yields th...

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Veröffentlicht in:Genome Biol
Hauptverfasser: Wang, Yanhong, Zhou, Lifang, Tao, Rui, Liu, Nan, Long, Jie, Qin, Fengming, Tang, Wenling, Yang, Yang, Chen, Qiang, Yao, Shaohua
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2020
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7453718/
https://ncbi.nlm.nih.gov/pubmed/32859244
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02137-6
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