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Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases
The tyrosine-type site-specific DNA recombinase Cre recombines its target site, loxP, with high activity and specificity without cross-recombining the target sites of highly related recombinases. Understanding how Cre achieves this precision is key to be able to rationally engineer site-specific rec...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7438526/ https://ncbi.nlm.nih.gov/pubmed/32814809 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-70867-5 |
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