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A cytometry-based assay to determine optimal Janelia Fluor dye labelling of Halo and SNAP tags

When used in combination, self-labelling protein tags such as Halo, SNAP, and CLIP allow for the simultaneous visualization of proteins across a wide fluorescence spectrum. However, the combination of cell type, ligand binding and fluorescent dye chemistry introduces several variables that need to b...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:J Biomol Tech
Päätekijät: Schaefer, Kathy, Xie, Frank (Liangqi), Weis-Garcia, Frances, White, Nicole
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Association of Biomolecular Resource Facilities 2020
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7424637/
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